T’s Apothecary

Specimen collection.

Environmental Science students have been working on a wide range of term projects. They’re required to use real data. Some are using the long term weather, climate and socioeconomic records from national and international data repositories. Others are collecting their own measurements — the ability to connect temperature, pH, and conductivity sensors to the new calculators have proven invaluable.

One project that I’ve been particularly happy that someone has taken up, because of its potential future use, has been to assemble a specimen collection cataloging the vegetative biodiversity in the area around the creek. With the help of TFS parent Scott Woodbury, who works for the Missouri Botanical Gardens, she’s collected, identified, and preserved dozens of specimens. She’s also compiled them all into an online phylogenetic tree (using mind42) that should serve as a wonderful reference for future class and student projects.

Life in Four Domains

The four domains of life, according to Boyer et al. (2010).

This wonderful, impressionistic image shows representatives of the three domains of life and large viruses, the proposed fourth.

This figure represents the living species in the four small pictures according to the current classification of organisms: eukaryotes (represented by yellow cell), bacteria (represented by green cell), Archaea (represented by blue cell) and viruses (represented by magenta colored Mimivirus).

Boyer et al. (2010): Boyer M, Madoui M-A, Gimenez G, La Scola B, Raoult D (2010) Phylogenetic and Phyletic Studies of Informational Genes in Genomes Highlight Existence of a 4th Domain of Life Including Giant Viruses. PLoS ONE 5(12): e15530. doi:10.1371/journal.pone.0015530

Carl Zimmer has an excellent piece in Discover Magazine that summarizes the research, and sets out the new tree of life. Particularly important, is the fact that viruses can transfer genes with each other. The other domains tend to mix their genes during reproduction.

Human Evolution not Drawn as a Tree?

Razib Kahn has a fascinating interview with Milford Wolpoff, one of the main scientists behind the research that argues that humans are not all part of a single family tree, descended from a single ancestor who moved out of Africa about 200,000 years ago.

This section focuses on the theory, and has a nice explanation of what mitochondrial DNA is (and why it’s important):

It gives an excellent perspective on how science works, and how scientists work (scientists are people too with all the problems that entails).

The entire thing is a bit dense, but it’s one of the better discussions I’ve seen describing the process of science in action, with little hints at all the challenges that arise from personality conflicts and competing theories.

Tree of life

One of the easiest and most elegant ways of explaining the classification of organisms, the history of life on Earth, and the relationships between different organisms is to construct a phylogenetic tree. I have a great exercise I like that takes just some bits of colored paper, string, a poster board and some thumbtacks.

To start, each student writes the Latin domain, kingdom, phylum, class, order, family, genus and species names on separate pieces of colored paper. I hand out paper in stacks and give them strict instructions not to rearrange the order of the colors. Wikipedia is actually a great resource for this because they tend to be quite reliable on this if they have the specie you’re looking for (and they have quite a bit).

Students then tape the pieces of paper together on a string, species at the bottom, domain at the top, and, one by one, tack them to the poster board. As each student attaches their string to the board they say the common name of their organism and then recite the phylogeny.

When I did the exercise on Monday, I asked the students to use the organisms they’re working on for their independent research projects so everything started with the domain Eukaria. Interestingly enough, the Wikipedia pages don’t have the domain classification, probably because they think it’s too obvious, but I had a number of kids spend quite a bit of time trying to figure it out; they probably benefited from doing so I didn’t mind at all.

Constructing the phylogenetic tree.

Classifications that are the same are tacked one on top of the other, Eukaria on top of Eukaria, Mammalia on top of Mammalia and so on, so that, as students add their parts of the phylogeny, you begin to see the phylogenetic tree. We had insects, mammals, plants and reptiles, so there was quite a nice variety represented.

After about half a dozen lineages were on the board, the procedure began to get a bit repetitive, so I started to ask students to guess, based on the common name, where the next species to go on would diverge from the rest of the emerging tree. Students seemed to like this part of it. I had started with homo sapiens when I demonstrated the procedure so it was salutary for them to see how much the other organisms differed from humans.

When everything is tacked on, you end up with a cute picture of a the tree of life that makes a cute, but awfully real looking, phylogenetic tree. Students tack their pieces of paper on the string at different distances, some much closer together than others. As a result, the final tree is looks as though it shows the genetic divergence between the different groups. It a fake, but lends a sense of verisimilitude non the less.